Complex formation between transfer RNAs with complementary anticodons: Use of matrix bound tRNA

It is shown that yeast tRNAPhe, chemically coupled by its oxidized 3′CpCpA end behaves exactly as free tRNAPhe in its ability to form a specific complex with E. coli tRNA2Glu having a complementary anticodon. The results support models of tRNA in which the 3′CpCpAOH end and the anticodon are not closely associated in the tertiary structure, and provide a convenient tool of general use to characterize others pairs of tRNA having complementary anticodons, as well as for highly selective purification of certain tRNA species. © 1973.SCOPUS: ar.j