Corticotropins (ACTH). XV. The action of chymotrypsin on α-corticotropin

The following peptide fragments have been isolated from chymotryptic digests of α-corticotropin and identified: Ser.Tyr; Ser(Tyr,Ser,Met,Glu)His.Phe; Arg.Try; Arg(Try,Gly.Lys,Pro,Val); Gly(Lys,Pro)Val.Gly.Lys; Lys(Arg 2,Pro)Val; Lys(Glu 2,Asp 2,Ala 2,Val,Tyr,Pro,Gly,Ser)GluNH 2.Ala.Phe; Ala(Phe,Pro)Leu; and Glu.Phe. These sequences are consistent with the proposed structure for α-corticotropin. One of the two amide groups in the peptide hormone has been located as glutamine at amino acid position 33. The specificity of chymotryptic hydrolysis of peptide bonds has been discussed.SCOPUS: ar.j