Variability of the reverse transcription step: practical implications
- Bustin, Stephen A.
- Dhillon, Harvinder S.
- Kirvell, Sara
- Greenwood, Christina
- Parker, Michael
- Shipley, Gregory L.
- Nolan, Tania
Publication date
January 2015
DOI Publisher
American Association for Clinical Chemistry (AACC)Abstract
The reverse transcription (RT) of RNA to cDNA is a necessary first step for numerous research and molecular diagnostic applications. Although RT efficiency is known to be variable, little attention has been paid to the practical implications of that variability
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