Extrachromosomally replicating viral DNA is usually free of cytosine methylation and viral templates methylated in vitro are poor substrates when used in replication assays. We have investigated the mechanism of inhibition of viral replication by DNA methylation using as a model the DNA A of African cassava mosaic virus. We have constructed two component helper systems which allow for separation of the transcriptional inhibition of viral genes necessary for replication from replication inhibition due to altered interaction between the replication complex and methylated viral DNA. Our results suggest that methylation-mediated reduction of viral replication is due to both repression mechanisms and that this provides two independent selection ...
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mamma...
14 p.-6 fig.DNA methylation is an important epigenetic mechanism for controlling innate immunity aga...
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mamma...
Extrachromosomally replicating viral DNA is usually free of cytosine methylation and viral templates...
Cytosine methylation is an epigenetic mark that promotes gene silencing and plays an important role ...
Bhendi yellow vein mosaic virus (BYVMV) belongs to the monopartite begomovirus associated with the &...
Although several hypomethylating agents such as 5-azadeoxycytidine and 5-fluorodeoxycytidine have be...
DNA methylation is a fundamental epigenetic modification that regulates gene expression and represse...
Similarly to other viruses, polyomaviruses require for their successful replication enzymes and othe...
The apparent simplicity of viruses hides the complexity of their interactions with their hosts. Viru...
One of the main questions of modern biology is how our cells interpret our genetic and epigenetic in...
Plants have evolved multiple mechanisms to respond to viral infection. These responses have been stu...
AbstractIntact clones containing partial repeats of the genomic components of African cassava mosaic...
The N6-methyladenosine (m(6)A) modifications in both viral and host cell RNAs play an important role...
The capacity for autonomous replication of the DNA A of African cassava mosaic virus (ACMV), a membe...
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mamma...
14 p.-6 fig.DNA methylation is an important epigenetic mechanism for controlling innate immunity aga...
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mamma...
Extrachromosomally replicating viral DNA is usually free of cytosine methylation and viral templates...
Cytosine methylation is an epigenetic mark that promotes gene silencing and plays an important role ...
Bhendi yellow vein mosaic virus (BYVMV) belongs to the monopartite begomovirus associated with the &...
Although several hypomethylating agents such as 5-azadeoxycytidine and 5-fluorodeoxycytidine have be...
DNA methylation is a fundamental epigenetic modification that regulates gene expression and represse...
Similarly to other viruses, polyomaviruses require for their successful replication enzymes and othe...
The apparent simplicity of viruses hides the complexity of their interactions with their hosts. Viru...
One of the main questions of modern biology is how our cells interpret our genetic and epigenetic in...
Plants have evolved multiple mechanisms to respond to viral infection. These responses have been stu...
AbstractIntact clones containing partial repeats of the genomic components of African cassava mosaic...
The N6-methyladenosine (m(6)A) modifications in both viral and host cell RNAs play an important role...
The capacity for autonomous replication of the DNA A of African cassava mosaic virus (ACMV), a membe...
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mamma...
14 p.-6 fig.DNA methylation is an important epigenetic mechanism for controlling innate immunity aga...
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mamma...