Laboratory generated bitumen fumes under standardized conditions: clean-up scheme and ion trap GC-MS analysis of VOC, semi-volatile and particulate PAH and PASH

This article reports the applicability of an efficient clean-up and enrichment procedure for the determination of polycyclic aromatic compounds (PAC) including polycyclic aromatic hydrocarbons (PAH) and polycyclic aromatic sulfur heterocyclic hydrocarbons (PASH) in the gas and particulate phases in raw bitumen and bitumen fumes by gas chromatography-ion trap mass spectrometry. The scheme includes a liquid-liquid partition to eliminate the aliphatics, a solid phase extraction on SiO2 cartridge to remove the polar compounds and an HPLC fractionation to isolate the aromatic fraction. The purified extract enables the determination of PAH profile from 2 to 7 rings including their methyl derivatives and 3 carcinogenic benzonaphthothiophene isomers. The reproducibility of the method was estimated by analyzing 6 bitumen replicates and is around the 30% overall uncertainty or less. In bitumen fumes generated at 170°C under standardized conditions, VOC and semi-volatile PAH were sampled with XAD-2 tubes and were also determined in six replicates. The VOCs are mainly monoaromatic derivatives (4% of fumes) and aliphatics essentially from C6 to C17 (27%). With regards to the particulate phase PAC, the coefficients of variation from 6 replicates are 5-24% for PAH and 6-10% for PASH. Data on VOC and PASH represent new contribution in the characterization of bitumen fumes. [Authors]]]> Polycyclic Hydrocarbons, Aromatic; Gas Chromatography, Mass Spectrometry; Spectrometry, Mass, Electrospray Ionization eng oai:serval.unil.ch:BIB_70207CBD9621 2022-05-07T01:20:11Z <oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:xs="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"> https://serval.unil.ch/notice/serval:BIB_70207CBD9621 La tuberculose extrapulmonaire [Extrapulmonary tuberculosis]. info:doi:10.1016/j.rmr.2011.05.021 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rmr.2011.05.021 info:eu-repo/semantics/altIdentifier/pmid/22542414 Mazza-Stalder, J. Nicod, L. Janssens, J.P. info:eu-repo/semantics/review article 2012 Revue des Maladies Respiratoires, vol. 29, no. 4, pp. 566-578 info:eu-repo/semantics/altIdentifier/eissn/1776-2588 urn:issn:0761-8425 <![CDATA[Extrapulmonary tuberculosis represents an increasing proportion of all cases of tuberculosis reaching 20 to 40% according to published reports. Extrapulmonary TB is found in a higher proportion of women, black people and immunosuppressed individuals. A significant proportion of cases have a normal chest X-Ray at the time of diagnosis. The most frequent clinical presentations are lymphadenitis, pleuritis and osteoarticular TB. Peritoneal, urogenital or meningeal tuberculosis are less frequent, and their diagnosis is often difficult due to the often wide differential diagnosis and the low sensitivity of diagnostic tests including cultures and genetic amplification tests. The key clinical elements are reported and for each form the diagnostic yield of available tests. International therapeutic recommendations and practical issues are reviewed according to clinical presentation