Can anodic aluminium oxide nanomembranes treated with nanometre scale hydroxyapatite be used as a cell culture substrate

In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types