Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a stable complex, known as the intasome1, 2. How the intasome interfaces with chromosomal DNA, which exists in the form of nucleosomal arrays, is currently unknown. Here we show that the prototype foamy virus (PFV) intasome is proficient at stable capture of nucleosomes as targets for integration. Single-particle cryo-electron microscopy reveals a multivalent intasome–nucleosome interface involving both gyres of nucleosomal DNA and one H2A–H2B heterodimer. While the histone octamer remains intact, the DNA is lifted from the surface of the H2A–H2B heterodimer to allow integration at strongly preferred superhelix location ±3.5 positions. Amino a...
Retroviral integration, the process of covalently inserting viral DNA into the host genome, is a poi...
Retroviral integrase (IN) functions within the intasome nucleoprotein complex to catalyze the insert...
A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy of t...
Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a...
Retroviral integrase can efficiently utilise nucleosomes for insertion of the reverse-transcribed vi...
Eukaryotic DNA binding proteins must access genomic DNA that is packaged into chromatin in vivo. Dur...
Eukaryotic DNA binding proteins must access genomic DNA that is packaged into chromatin in vivo. Dur...
To establish productive infection, a retrovirus must insert a DNA replica of its genome into host ce...
BACKGROUND: Retroviral integration depends on the interaction between intasomes, host chromatin and ...
Retroviral DNA integration takes place in the context of the intasome nucleoprotein complex. X-ray c...
The interactions between a retrovirus and host cell chromatin that underlie integration and provirus...
The ability of retroviruses to integrate their genomes into host chromatin is a key step for the com...
Retroviral integration, the process of covalently inserting viral DNA into the host genome, is a poi...
Retroviral integrase (IN) functions within the intasome nucleoprotein complex to catalyze the insert...
A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy of t...
Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a...
Retroviral integrase can efficiently utilise nucleosomes for insertion of the reverse-transcribed vi...
Eukaryotic DNA binding proteins must access genomic DNA that is packaged into chromatin in vivo. Dur...
Eukaryotic DNA binding proteins must access genomic DNA that is packaged into chromatin in vivo. Dur...
To establish productive infection, a retrovirus must insert a DNA replica of its genome into host ce...
BACKGROUND: Retroviral integration depends on the interaction between intasomes, host chromatin and ...
Retroviral DNA integration takes place in the context of the intasome nucleoprotein complex. X-ray c...
The interactions between a retrovirus and host cell chromatin that underlie integration and provirus...
The ability of retroviruses to integrate their genomes into host chromatin is a key step for the com...
Retroviral integration, the process of covalently inserting viral DNA into the host genome, is a poi...
Retroviral integrase (IN) functions within the intasome nucleoprotein complex to catalyze the insert...
A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy of t...