Quantitative measurement of L1 HPV16 methylation for the prediction of pre-invasive and invasive cervical disease
- Kottaridi, C
- Kyrgiou, M
- Pouliakis, A
- Magkana, M
- Aga, E
- Spathis, A
- Mitra, A
- Makris, G
- Chrelias, C
- Mpakou, V
- Paraskevaidis, E
- Panayiotides, J
- Karakitsos, P
DOIAbstract
Background: Methylation of the HPV DNA has been proposed as a novel biomarker. Here, we correlated the mean methylation level of 12 CpG sites within L1 gene, to the histological grade of cervical precancer and cancer. We assessed whether HPV L1 gene methylation can predict the presence of high-grade disease at histology in women testing positive for HPV 16 genotype. Methods: Pyrosequencing was used for DNA methylation quantification and 145 women were recruited. Results: We found that the L1 HPV16 mean methylation (+/-SD) significantly increased with disease severity [CIN3=17.9%(±7.2) vs CIN2=11.6%(±6.5), p<0.001 or vs CIN1 =9.0%(±3.5), p<0.001). Mean methylation was a good predictor of CIN3+ cases; the Area Under the Curve (AUC) was higher...
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