Effect of pyridine triphenyl borane (KH101) on the reproduction of Calanoid Copepod, Acartia omorii Bradford

Research PaperCurrently documented that pyridine triphenyl borane (KH101) is possible to use as an alternative of tributyltin based antifouling biocide in coastal and marine environment. But its impact on marine life especially on smaller zooplankton is so far unknown. The present study was conducted to determine the effect of KH101 on survival, egg production and egg hatch of a calanoid copepod, Acartia omorii under bath administration. Survival was observed every 24h for 10 days. Each 10 individuals of adult female were incubated at different concentrations of KH101 (0.001, 0.01, 0.1, 1.0, 10.0 and 100.0μg/L) with food (Isochrysis sp. + Tetraselmis sp., ratio 1:1). Egg production was examined through the incubation of single gravid female with above conditions but only in 0.001, 0.01, 0.1 and 1μg/L of KH101 concentrations. Egg hatching success was determined for each 60 eggs at different concentration of KH101, which were obtained from pre-exposed female at the same concentration. Statistically no discrepancy was observed in control and solvent control (10ml/L, dimethyl sulphoxide) treatment in all observations. LC₅₀s were obtained in 0.1, 1.0, 10.0 and 100.0μg/L concentrations of KH101 after 168, 96, 48 and 24h, respectively. Significant (P