The identification of ubiquitin-mediated proteolysis (UMP) substrates and of the mechanism of their polyubiquitination is important to understanding how UMP regulates cell cycle progression. To study the mechanism of substrate selection, a colorimetric assay using expression of Ade2p to complement the red ade colony phenotype was developed for the visualization of protein levels in the yeast Saccharomyces cerevisiae. Fusion protein levels are visualized as differences in yeast colony color and prototrophy: stable fusion proteins produce white ADE colonies and unstable fusions produce red ade colonies. Yeast transformants expressing Ade2p fused to the UMP-target proteins Clb2p and Pds1p form red colonies and do not accumulate the fusion prot...
Endocytosis is a fundamental process for internalizing material from the plasma membrane, including ...
Ubiquitination plays an important role in many cellular processes and is implicated in many diseases...
In Saccharomyces cerevisiae, several of the proteins involved in the Start decision have been identi...
A large pool of proteins localizes in the cytosol of eukaryotic cells. Proteins in the cell can be m...
Since its discovery nearly 40 years ago, many components of the ubiquitin-proteasome system (UPS) ha...
AbstractClb2 mitotic cyclin inhibits cell cycle progression by preventing mitotic exit and DNA synth...
The purpose of protein homeostasis (proteostasis) is to maintain proteome integrity, thereby promoti...
Our studies of the yeast ubiquitin-proteasome pathway have uncovered a number of general principles ...
<p><b>A</b>, Schematic representation of substrates used for (<b>B</b>). <b>B</b>, Stability of prot...
A library of random 10 residue peptides fused to the N-terminus of a reporter protein was screened i...
A library of random 10 residue peptides fused to the N-terminus of a reporter protein was screened i...
As a part of protein quality control (PQC), the ubiquitin-proteosome system (UPS) ubiquitinates and ...
Ubiquitin-mediated proteolysis is a major pathway for selective protein degradation in eukaryotic ce...
The DNA damage checkpoint is a signal transduction pathway that is evolutionarily conserved from yea...
<p>Cellular transitions are achieved by the concerted actions of regulated degradation pathways. In ...
Endocytosis is a fundamental process for internalizing material from the plasma membrane, including ...
Ubiquitination plays an important role in many cellular processes and is implicated in many diseases...
In Saccharomyces cerevisiae, several of the proteins involved in the Start decision have been identi...
A large pool of proteins localizes in the cytosol of eukaryotic cells. Proteins in the cell can be m...
Since its discovery nearly 40 years ago, many components of the ubiquitin-proteasome system (UPS) ha...
AbstractClb2 mitotic cyclin inhibits cell cycle progression by preventing mitotic exit and DNA synth...
The purpose of protein homeostasis (proteostasis) is to maintain proteome integrity, thereby promoti...
Our studies of the yeast ubiquitin-proteasome pathway have uncovered a number of general principles ...
<p><b>A</b>, Schematic representation of substrates used for (<b>B</b>). <b>B</b>, Stability of prot...
A library of random 10 residue peptides fused to the N-terminus of a reporter protein was screened i...
A library of random 10 residue peptides fused to the N-terminus of a reporter protein was screened i...
As a part of protein quality control (PQC), the ubiquitin-proteosome system (UPS) ubiquitinates and ...
Ubiquitin-mediated proteolysis is a major pathway for selective protein degradation in eukaryotic ce...
The DNA damage checkpoint is a signal transduction pathway that is evolutionarily conserved from yea...
<p>Cellular transitions are achieved by the concerted actions of regulated degradation pathways. In ...
Endocytosis is a fundamental process for internalizing material from the plasma membrane, including ...
Ubiquitination plays an important role in many cellular processes and is implicated in many diseases...
In Saccharomyces cerevisiae, several of the proteins involved in the Start decision have been identi...