TIME-RESOLVED RESONANCE RAMAN ($TR^{3}$) SPECTROSCOPY

$^{1}$R. B. Srivastava, M. W. Schuyler, L. R. Dosser, F. J. Purcell and G. H. Atkinson (submitted to Chem, Phys. Letters). This work was supported by the NSF.""Author Institution: Department of Chemistry, Syracuse UniversityExperimental techniques designed to obtain resonance Raman spectra of short-lived intermediates during photolytically-induced reactions will be described. These methods use high peak power ($>$ 1 MW), pulsed laser excitation to generate Raman scattering and intensified vidicons as multi-wavelength detectors. The time resolution available extends from $10^{-8}$ to several seconds. Studies of labile biophysical systems (oxyhemoglobin and cytochrome c) have demonstrated that resonance Raman spectra obtained with such high peak-power excitation exhibit no saturation or denaturation $effects^{1}$ Results on the reaction intermediates in the ligation of hemoglobin and the oxidative photocyclization of substituted amines to carbazoles will be presented to illustrate the capabilities of these techniques