A non-intrusive digital image analyses technique is applied to study size driven segregation of a binary mixture of coloured glass beads in a bubbling gas-fluidised bed. Segregation rates and patterns obtained from experiments are compared to numerical simulations performed with a two-dimensional multi-fluid Eulerian model, that uses closure laws according to the kinetic theory of granular flow. It is demonstrated that prediction of segregation is a rather severe test case for fundamental hydrodynamic models, since bubble dynamics and momentum transfer between particles of different classes have to be modelled correctly. At all gas velocities segregation rates predicted by the multi-fluid model were much higher than those observed in experi...