Two stage process of microalgae cultivation for starch and carotenoid production

Biotechnological processes based on microalgae cultivation are promising for several industrial applications. Microalgae are photoautotrophic microorganisms and can thus grow by using renewable and inexpensive resources as sunlight, inorganic salts, water and CO2. They can store high amounts of neutral lipids (bioil), carbohydrates (mainly starch), carotenoids (such as lutein, astaxanthin, beta-carotene), proteins and other molecules. Productions of lipids and carbohydrates have recently received an increasing interest for biofuel production, while proteins, carotenoids and other minor products are usable as feed additives and nutraceutical compounds. Biofuel production from microalgae is not yet economically sustainable, while there are different industrial plants in the world for the production of high values chemicals as carotenoids. Starch production from microalgae has been investigated mainly for the production of biofuels (e.g. bioethanol) by successive fermentation. However, purified starch can be used for other aims such as the production of bioplastics. Superior plants as corn, potato and wheat are currently used for this purpose. However, there are different environmental and economic issues related to the use of fertile lands and edible plants for these kinds of productions. Microalgae can solve these social and ethical issues because they can grow on nonfertile lands and also reach starch productivity per hectare higher than plants. In this work, the production of starch and carotenoids from Scenedesmus sp. microalgal strain is reported. A two-stage process has been developed in order to reduce operative and investment costs. In the first stage, microalgae are cultivated in photoautotrophic conditions and then, when biomass concentration rises and light becomes a limiting factor for growth, microalgae are transferred to a heterotrophic reactor. In this reactor, microalgae are cultivated by using wastewaters as source of nutrients (mainly organic carbon). Microalgae use organic carbon to synthesize starch and simultaneously reduce the content of pollutants in the wastewater (codepuration). Biomass separated by the culture medium is treated for the extraction of lipids containing different antioxidant carotenoids (such as astaxanthin and lutein) and starch granules as raw material for biopolymers