In this paper, a new analytical model is proposed to describe the behavior of uncertainty for a generic vision-based measurement system applied to mechanical vibration measurement. In particular, this paper proposes a novel way to evaluate the effects of motion blur. The theoretical framework presented here takes into account the camera acquisition parameters, the dynamics of the measurand (in terms of instantaneous speed), and the image scaling factor to develop a physical model of uncertainty when measuring a mainly monomodal vibration. Uncertainty is evaluated starting from the normalized discrepancy between a measured position and the actual one. The analytical model proposed here has been validated extensively with the help of a test s...