Thermally induced conformational transitions of three proteins of increasing intrinsic disorder-cytochrorne c, the tumor suppressor protein p53 DNA binding domain (p53 DBD), and the N-terminus of the oncoprotein murine double minute 2 (NT-MDM2) have been studied by native mass spectrometry and variable-temperature drift time ion mobilitY mass spectrometry (VT-DT-IM-MS). Ion mobility measurements were carried out at temperatures ranging from 200 to 571 K. Multiple conformations are observable over several charge states for all three monomeric proteins, and for cytochrome c, dimers of significant intensity are also observed. Cytochrome c [M + 5H](5+) ions present in one conformer of CCS similar to 1200 angstrom(2), undergoing compaction in li...
The precise mechanism of protein folding remains elusive and there is a deficiency of biophysical te...
The global dimensions and amplitudes of conformational fluctuations of intrinsically disordered prot...
Ion mobility spectrometry – mass spectrometry (IMS-MS) based techniques have significantly advanced ...
Thermally induced conformational transitions of three proteins of increasing intrinsic disordercyto...
Analysis and characterisation of biomolecules using mass spectrometry has advanced over the past dec...
Symmay The tumour-suppressor protein p53 is a metal-binding transcription factor with sequence-speci...
The thermal stability and strength of interactions in proteins are commonly measured using isotherma...
Native mass spectrometry is a powerful tool for the analysis of noncovalent complexes. When coupled ...
This work uses electrospray ionization mass spectrometry (ESI-MS) in conjunction with hydrogen/deute...
Intrinsically disordered proteins have been reported to undergo ‘disorder to order’ transitions upon...
The link between structure and function of a given protein is a principal tenet of biology. The esta...
Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions ...
Integrative structural biology employs not only high-resolution techniques for the determination of ...
The initial stages of protein unfolding may reflect the stability of the entire fold and can also re...
This dissertation presents efforts to expand the role of mass spectrometry (MS) in structural biolog...
The precise mechanism of protein folding remains elusive and there is a deficiency of biophysical te...
The global dimensions and amplitudes of conformational fluctuations of intrinsically disordered prot...
Ion mobility spectrometry – mass spectrometry (IMS-MS) based techniques have significantly advanced ...
Thermally induced conformational transitions of three proteins of increasing intrinsic disordercyto...
Analysis and characterisation of biomolecules using mass spectrometry has advanced over the past dec...
Symmay The tumour-suppressor protein p53 is a metal-binding transcription factor with sequence-speci...
The thermal stability and strength of interactions in proteins are commonly measured using isotherma...
Native mass spectrometry is a powerful tool for the analysis of noncovalent complexes. When coupled ...
This work uses electrospray ionization mass spectrometry (ESI-MS) in conjunction with hydrogen/deute...
Intrinsically disordered proteins have been reported to undergo ‘disorder to order’ transitions upon...
The link between structure and function of a given protein is a principal tenet of biology. The esta...
Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions ...
Integrative structural biology employs not only high-resolution techniques for the determination of ...
The initial stages of protein unfolding may reflect the stability of the entire fold and can also re...
This dissertation presents efforts to expand the role of mass spectrometry (MS) in structural biolog...
The precise mechanism of protein folding remains elusive and there is a deficiency of biophysical te...
The global dimensions and amplitudes of conformational fluctuations of intrinsically disordered prot...
Ion mobility spectrometry – mass spectrometry (IMS-MS) based techniques have significantly advanced ...