Plant WEE1 Kinase Interacts With a 14-3-3 Protein, GF14w(omega) but a Mutation of WEE1 at S485 Alters Their Spatial Interaction

In animals, 14-3-3 proteins bind two cell cycle proteins WEE1 and CDC25 stabilising their phosphorylated\ud state. We report here for the first time interactions between WEE1 and 14-3-3 proteins both in vitro and in vivo in plants.\ud The Arabidopsis 14-3-3 family partitions into either an Epsilon or Non-Epsilon group. In a yeast 2-hybrid screen\ud Arabidopsis WEE1 interacted with the Non-Epsilon group. Subsequently, we focussed on Non-Epsilon GF1