3-D volumetric Lensfree imaging using Digital Holographic Microscopy

Despite of advancement in microscopic techniques most of the\ud improved microscopy modalities are complex, bulky and require costly setup. Many of the modalities are not capable of live imaging.Today imaging devices are needed to be compact, cost-effective, portable, light-weight and significantly accurate and easy to handle. Digital holography has overcome few barriers but still demands costly setup. Here we demonstrate the lens free imaging which is based on “Digital In-line holography” principle and does not require bulky and costly setup. The technique uses Light emitting diode (LED) as a source of light and CCD camera to capture the hologram of the object which allow to reconstruct the image using numerical algorithm on computing machines. This technique has many applications including one in telemedicine and can address the issues related to the rural health