Exogenous Glucose Administration Impairs Glucose Tolerance and Pancreatic Insulin Secretion during Acute Sepsis in Non-Diabetic Mice

Objectives: The development of hyperglycemia and the use of early parenteral feeding are associated with poor\ud outcomes in critically ill patients. We therefore examined the impact of exogenous glucose administration on the\ud integrated metabolic function of endotoxemic mice using our recently developed frequently sampled intravenous\ud glucose tolerance test (FSIVGTT). We next extended our findings using a cecal ligation and puncture (CLP) sepsis\ud model administered early parenteral glucose support.\ud Methods: Male C57BL/6J mice, 8-12 weeks, were instrumented with chronic indwelling arterial and venous\ud catheters. Endotoxemia was initiated with intra-arterial lipopolysaccharide (LPS; 1 mg/kg) in the presence of saline or\ud glucose infusion (100 μL/hr), and an FSIVGTT was performed after five hours. In a second experiment, catheterized\ud mice underwent CLP and the impact of early parenteral glucose administration on glucose homeostasis and mortality\ud was assessed over 24 hrs.\ud Measurements: And MAIN RESULTS: Administration of LPS alone did not impair metabolic function, whereas\ud glucose administration alone induced an insulin sensitive state. In contrast, LPS and glucose combined caused\ud marked glucose intolerance and insulin resistance and significantly impaired pancreatic insulin secretion. Similarly,\ud CLP mice receiving parenteral glucose developed fulminant hyperglycemia within 18 hrs (all > 600 mg/dl) associated\ud with increased systemic cytokine release and 40% mortality, whereas CLP alone (85 ± 2 mg/dL) or sham mice\ud receiving parenteral glucose (113 ± 3 mg/dL) all survived and were not hyperglycemic. Despite profound\ud hyperglycemia, plasma insulin in the CLP glucose-infused mice (3.7 ± 1.2 ng/ml) was not higher than sham glucose\ud infused mice (2.1 ± 0.3 ng/ml).\ud Conclusions: The combination of parenteral glucose support and the systemic inflammatory response in the acute\ud phase of sepsis induces profound insulin resistance and impairs compensatory pancreatic insulin secretion, leading\ud to the development of fulminant hyperglycemia