Efficient protocol for micropropagation of Calamus huegilianus an endangered taxon.

Calamus huegilianus is a rare and endangered taxon of the Western ghats, India. Shoot tips of three month old seedlings were inoculated onto MS and L2 media supplemented with various growth regulators to record their morphogenetic potential. Both direct and indirect organogenesis was recorded depending on the type and concentration of hormones supplemented to the media. L2+BAP (2mg/l) and L2+NAA (2mg/l) + BAP (4mg/l) were proved to be the best combination for direct and indirect regeneration. Thus obtained shoots were rooted on L2 supplemented with either IAA or NAA. Acclimatized regenerates in vermiculate and perlite were transferred to field. Nearly 40% of survival was recorded