Characterization of esterolytic activity from edible mushroom Hypsizygus ulmarius

Characterization of esterase activity from the edible mushroom Hypsizygusulmariuswere investigated. The ext. prepd. from the mushroom sample showed the esterolytic activity. The ext. had the highest activity in 2mM of 1-​naphthyl acetate as a substrate. pH and temp. optima were found to be at 6.0 and 30°C. The pH and temp. stability profiles show that this enzyme is more stable under acidic, neutral and basic conditions and stable up to 40°C. The Km and Vmax of esterase enzyme acting on 1-​naphthyl acetate were 0.33 and 0.47mM. The effect of alternate product (p-​nitrophenol) on esterase activity were found to be at 10mM. The effect of metals on activity of esterase increased with the addn. of CuSO4 and decreased with FeSO4