A protocol for in vitro regeneration of Eryngium foetidum L.

Eryngium foetidum L. is an important plant cultivated as leafy vegetable and for its essential oil, which is of high economical value in international market. Multiple shoots (17.8) were obtained through direct regeneration from leaf excised from field grown plants of E. foetidum and cultured on Linsmaier and Skoog's Basal Medium (LSBM) fortified with BAP (1.5 mgl-1) and PVP (250 mgl-1). After subculture on the same medium, the multiple shoots formed roots, thereby eliminating an additional step of in vitro rooting. The well-developed plants were hardened and successfully transferred to field with 88 survival frequency