An Analysis of Pre-B Cell Lymphoblastic Lymphoma of the skin by Using 3-Color flow Cytometry

A Trial of Application of the Updated Kiel Classification to Extranodal Malignant Lymphomas with Advice of K. Lennert and AC Feller : Proceedings of the Second Seminar on Malignant Lymphoma in Kagoshima, Japan. April 13 - 15, 1994A case of CD45-negative, CDlO-positive lymphoblastic lymphoma (LBL) with precursor B lymphocyte (pre-B) phenotype, was analyzed by using 3 color flow cytometry (3-FCM) in addition to morphological observation and immunohistochemistry. The difference of antigen expression as defined by the fluorescent intensity of FCM was significantly clear in CD20 and CD22 between lymphoma cells and normal B lymphocyte. Thus, the lymphoma cell population could be cleary separated on the three dimensional cubic representation using PAINT-A-GATE software. Also, cytoplasmic IgM was easily demonstrated by the combined use of both flow cytometry and immunohistochemistry. The former showed negative and the latter positive for IgM. For a better clinical application of the detailed phenotypic results examined by 3-FCM, it could be considered that the unusual expression or alternation of B cell-lineage antigens and defectiveness of common antigen may be useful to detect small number of lymphoma cells in peripheral blood or bone marrow