Highly Efficient Preparation of Bisphenol A Glucuronide

Bisphenol A(BPA), an industrial chemical, has been present in many hard plastic bottles, metal-based food and beverage cans. Many governments are trying to reduce the human exposure to BPA because it is really a readily accessible toxin for human. BPA can be extensively metabolized to bisphenol A-beta-O-glucuronide(BPA-G) by UDP-glucuronosyltransferases(UGTs), thus the biological activities of BPA-G should be studied intensively. However, the studies on BPA-G are very limited due to the difficulty for preparation of BPA-G. In this work, the BPA-G was prepared using BPA as the starting material and rat liver microsomes (RLM) as the enzyme source. A series of orthogonal experiments was designed and performed to determine. the optimal reaction conditions for BPA glucuronidation in RLM. After incubation under the optimal reaction conditions, the conversion of BPA was higher than 98%. A unique solid-phase extraction column (SPE) packed with C-18 WAX was used to enrich and purify the product BPA-G with high yield. Finally, the product was identified by both ESI-MS and NMR techniques