Synthesis and application of photoaffinity probe containing an intact isoprenoid chain

Two novel chemical probes each carrying an intact isoprenoid chain, a biotin tag and a benzophenone moiety were synthesized. Photoaffinity labeling of the Saccharomyces cerevisiae cell lysate revealed that these probes could selectively trap some proteins, and proteins with molecular weight of similar to 70 KDa appeared as a major band upon Streptavidin blot analysis. (C) 2009 Elsevier Ltd. All rights reserved