Matrix molecularly imprinted polymer for enriching lysozyme in protein samples

The effects of the molar ratio of functional monomer to template protein, the pH and the ionic strength of the medium on the Interaction between the functional monomers and template protein were primarily investigated from the molecular level by UV spectrophotometry. Then lysozyme(Lyz) -imprinted polyacrylamide gel was prepared to recognize effectively Lyz in protein samples. With acrylamide and N,N-methylenediacryl-amide as the polymeric matrix and sillca as the pore-forming reagent, after template and silica were removed, the surface of gel polymer had a large quantity of well-distributed macropores, which were suited to let the proteins pass in and out. The recognition and selectivity of the imprinted polymers were studied with equilibrium adsorption. Selective adsorption experiments of the imprinted gel show that imprinted gel exhibited good recognition capability for Lyz compared with bovine serum albumin or ovalbum as the completive substance