Analysis of triterpenoids in fruiting bodies of ganoderma lucidum with off-line supercritical fluid extraction - High performance liquid chromatography system

An extraction method of triterpenoids in the fruiting bodies of ganoderma lucidum using supercritical fluid (SF) was developed. The effects of pressure, temperature and time were investigated with respect to extraction yield. The optimized parameters were as follows: extraction pressure 15 MPa, extraction temperature 35 degrees C, extraction time 120 min, flow rate of CO2 1 mL/min, and the temperature of backpressure regulator 50 degrees C. In addition, a gradient elution method of high performance liquid chromatography was developed for the analysis of extracted triterpenoids. By comparison, it found that the chromatograms of the triterpenoids extracted by supercritical fluid and methanol were quite similar to each other, which demonstrated that supercritical fluid could be used as a new generation of green extraction solvent instead of methanol