Solid-phase extraction of active component of Chinese traditional medicine fisetin by using molecularly imprinted polymers

Fisetin is the main function integrant in Cotinus coggygria scop of Chinese traditional medicine. Using fisetin as imprinted molecule, acrylamide as the functional monomer, and EDMA as the crosslinking agent, a polymer was prepared by molecular imprinting technique. The polymer was loaded into the self-made solid-phase extraction(SPE) cartridge. A SPE procedure was suggested for extracting and concentrating fisetin by using different volume fractions of ethanol in aqueous solution as the loading conditions. In order to optimize the washing and eluting solution, the adsorption of fisetin in different solutions was studied. It was found that the fisetin and analogue quercetin were separated primely