Selection of a first-dimensional separation method is very important in proteomic studies performed with two-dimensional liquid chromatography (2D-LC), since reversed-phase chromatography (RPLC) is usually chosen as the second-dimensional separation method in most studies. In this paper, we assess the potential use of mixed-mode reversed-phase/weak cation-exchange (RP/WCX) chromatography in 2D-LC proteomic studies. First, a new RP/WCX mixed-mode stationary phase (named C18WCX) was synthesized based on the polar-copolymerized approach, and a C18WCX column with separation efficiency comparative to conventional C18 columns was developed. This new mixed-mode column primarily provides hydrophobic interactions under acidic condition, but can offe...
The peak-production rate (peak capacity per unit time) in comprehensive off-line two-dimensional liq...
Over the years, two-dimensional Liquid Chromatography (2D-LC) has played a key role in the field of ...
A two-dimensional capillary liquid chromatography (LC) method is described which is based on microfr...
Offline two-dimensional chromatography is a common means to achieve deep proteome coverage. To reduc...
A comprehensive two-dimensional liquid chromatographic system (2D SCX/RP) is constructed with a 10-p...
Two-dimensional liquid chromatography (2D-LC) allows analysts to deal with complex samples that eith...
A novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography ...
Multidimensional peptide fractionation is widely used in proteomics to reduce the complexity of pept...
Multidimensional liquid-based separation techniques are described for maximizing the resolution of t...
In the pharmaceutical field, there is considerable interest in the use of peptides and proteins for ...
The general strategy in proteomic research consists in sample preparation, protein or peptide separa...
As all alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC fo...
Protein phosphorylation regulates a series of important biological processes in eukaryotes. However,...
Previously, we described an online high-/low-pH RP-RP LC system exhibiting high-throughput, automata...
To obtain the best compromise between peak capacity and analysis time in one-dimensional and two-dim...
The peak-production rate (peak capacity per unit time) in comprehensive off-line two-dimensional liq...
Over the years, two-dimensional Liquid Chromatography (2D-LC) has played a key role in the field of ...
A two-dimensional capillary liquid chromatography (LC) method is described which is based on microfr...
Offline two-dimensional chromatography is a common means to achieve deep proteome coverage. To reduc...
A comprehensive two-dimensional liquid chromatographic system (2D SCX/RP) is constructed with a 10-p...
Two-dimensional liquid chromatography (2D-LC) allows analysts to deal with complex samples that eith...
A novel two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography ...
Multidimensional peptide fractionation is widely used in proteomics to reduce the complexity of pept...
Multidimensional liquid-based separation techniques are described for maximizing the resolution of t...
In the pharmaceutical field, there is considerable interest in the use of peptides and proteins for ...
The general strategy in proteomic research consists in sample preparation, protein or peptide separa...
As all alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC fo...
Protein phosphorylation regulates a series of important biological processes in eukaryotes. However,...
Previously, we described an online high-/low-pH RP-RP LC system exhibiting high-throughput, automata...
To obtain the best compromise between peak capacity and analysis time in one-dimensional and two-dim...
The peak-production rate (peak capacity per unit time) in comprehensive off-line two-dimensional liq...
Over the years, two-dimensional Liquid Chromatography (2D-LC) has played a key role in the field of ...
A two-dimensional capillary liquid chromatography (LC) method is described which is based on microfr...