Development of a Comprehensive Two-Dimensional Liquid Chromatography / Mass Spectrometry System and its Applications in Analyzing Triglycerides

In the present work, an on-line comprehensive silver-ion liquid chromatography × reversed- phase liquid chromatography system was constructed to analyze triglycerides (TAGs). The column parameters and mobile phases were optimized. The system coupled with atmospheric pressure chemical ionization mass spectrometry (APCI MS) was applied to analyze an edible peanut oil and a mouse liver extract. TAG structures with different carbon chains were identified based on TAGs’ retention behaviors and their MS fragments. The results showed developed comprehensive two-dimensional LC system demonstrated unique selectivity for TAG compounds and satisfactory peak capacity.In the present work, an on-line comprehensive silver-ion liquid chromatography × reversed- phase liquid chromatography system was constructed to analyze triglycerides (TAGs). The column parameters and mobile phases were optimized. The system coupled with atmospheric pressure chemical ionization mass spectrometry (APCI MS) was applied to analyze an edible peanut oil and a mouse liver extract. TAG structures with different carbon chains were identified based on TAGs’ retention behaviors and their MS fragments. The results showed developed comprehensive two-dimensional LC system demonstrated unique selectivity for TAG compounds and satisfactory peak capacity