Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves
- Li, Yong
- Pang, Tao
- Li, Yanli
- Wang, Xiaolin
- Li, Qinghua
- Lu, Xin
- Xu, Guowang
- 许国旺
DOIAbstract
Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leavesA gas chromatography-mass spectrometric method was developed for profiling of tobacco leaves. The differentiation among tobacco leaves planted in two different regions was investigated. Prior to analysis, the extraction solvent formulation was optimized and a combination of water, methanol and acetonitrile with a volume ratio of 3:1:1 was found to be optimal. The reproducibility of the method was satisfactory. Kendall tau-b rank correlation coefficients were equal to 1 (p<0.05) for 82% of the resolved peaks (up to 95% of the overall peak areas), indicating the good response correlation. Forty-four compounds including 9 saccharides, 9 alcohols, 9 amino acid...
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