High-density cell cultivation for photobiological hydrogen production by marine green alga Platymonas subcordiformis

hydrogen production by Platymonas subcordiformis, the effects of kinetics parameters of algal cultures growninCO2-supplemented air bubble column bioreactor was investigated on H2 photoproduction regulated by carbonylcyanide m-chlorophenylhrazone (Guo et al., 2008;Guan et al., 2004a,b; Ran et al., 2006). To achieve the optimal hydrogen production, the 7-day old cells grown in 3% CO2-supplemented air bubble column bioreactor were concentrated to a final cell density of 8.0×106 cells ml−1 in the spentmedium. The sealed cultures were subjected to the dark anaerobic induction for 4 h. At the start of illumination for hydrogen production, the pH was readjusted to 7.5 and 15 M CCCP was added into the cultures to initiate the hydrogen photoproduction. As a result, hydrogen production has been significantly improved