Bacteriological and Immunopathological Diagnosis on Morel's Disease

Immunopathological studie on Morel's disease were carried out, using plaque forming cell assay (PFCA). About forty five samples taken from sheep abscesses for isolation the causative agent. Thirty two isolates were identified asStaphylococcus species; sixteen isolates were identified biochemically as Staphylococcus aureus subsp. anaerobius,nine isolates as Staphylococcus aureus, five isolates as Staphylococcus epidermidis, one isolates asStaphylococcus simians,and another one as Staphylococcus cohni subsp. urealyticus. The tissue samples collected from sheep with abscess disease for histopathology, showed areas of liquifactive necrosis surrounded by inflammatory cells and fibrous tissue capsule or collection of inflammatory cels predominantly neutrrophils and mononuclear cells surrounded by a weak fibrous reaction. Rats were found to be resistant toexperimental infection with Staphylococcus aureus subsp. anaerobius, Staphylococcus aureus, a combination of Staphylococcus aureus subsp. anaerobiousand Staphylococcus aureus, Staphylococcus caseolyticusand Staphylococcus lugdunensis. The rats were divided into 5groups each group consist of 3rats. Group 1 was inoculated by Staphylococcus aureus subspp. Anaerobiusand the plaque forming cells (PFCs) were found to be 6915. Group 2were inoculated by Staphylococcus aureusand the PFCs were found to be 4583. Group 3 were inoculated by a combination of Staphylococcus aureus subspp. anaerobiusand Staphylococcus aureus and the PFCs were found to be 14,738. 11 Group 4 were inoculatedby Staphylococcus caseolyticusand the PFCs were found to be 7536. Group 5 were inoculated by Staphlococcus lugdunensisand PFCs were found to be 3390. In this study, the production of plaques was observed in un tanned red blood cells compared with tanned red blood cells. Another important observation is that the synergetic reaction revealed more plaques formed as compared with the summation result of the combination between Staphylococcus aureus subspp. anaerobiusand Staphylococcus aureus. The rats in this study were resistant to experimental infection with Staphylococcus aureus subspp. anaerobius, S. aureus, S. aureus subspp. anaerobius + S. aureus, S. caseolyticus and S. lugdunensis. Beside the experiments, samples werecollected from naturally infected sheep with a disease for histopathological sections, showed mature abscess of liquifactive necrosis surrounded by inflammotry cells and capsule of fibres tissue, or as a collection of inflammotry cells predominantly neutrophils and mononuclear cells surrounded by a weak fibrosi