Identification And Phylogenetic Analysis Of Common 1 Pumpkin Viruses In Sudan

Identification And Phylogenetic Analysis Of Common 1 Pumpkin Viruses In SudanThe objective of this study was to identify viral species infecting pumpkin in Khartoum and 21 Gezira states, Sudan, and to evaluate the genetic variability among members of the same 22 identified species. A total of 60 symptomatic pumpkin leaf samples were collected from three 23 different locations, around Khartoum State, and examined for viral incidence. Symptoms 24 observed varied from leaf mosaic, malformation, blistering and stunting. Degenerate genus-25 specific and species-specific primers were used for the detection of common cucurbit viruses. 26 Results obtained showed that the highest incidence (21.7%) was for Cucurbit chlorotic yellows 27 virus (CCYV) and Cucurbit yellow stunting disorder virus (CYSDV), followed by (18.3%) 28 Zucchini yellow mosaic virus (ZYMV), and (15%) Watermelon chlorotic stunt virus (WmCSV). 29 Multiple infections with more than one virus were also detected. The partial nucleotide 30 sequences of the regions encoding for CI and CP, CP, Hsp70h, and AV2 proteins of ZYMV, 31 CYSDV, CCYV and WmCSV, respectively, have been deposited in the NCBI GenBank. 32 Phylogenetic analysis based on nucleotide sequences showed no or little variation among the 33 studied viral isolates since each is grouped well with its corresponding reference species