Somatic Embryogensis From Shoot Tip and Immature Inflorescence of Phoenix dactylifera L. cv. Mishrig Wad-laggai

Page(s): 8 (1), 88-99,11 RefIn-vitro culture of segments of leaf bases from shoot tips of the date palm cultivar Mishrig Wad-laggai on a medium containing high auxin concentration (100 mg/L 2,4-D) yielded a white nodular callus. Callus was maintained for 18 months by repeated subculture and reduction of auxin level to 50 mg/L. This resulted in three types of callus; namely, normal nodular callus, granular callus and aggregated clumps callus. Only the aggregated clumps callus gave plantlets upon transfer onto a medium containing 0.01 mg/L NAA. Different callus types were anatomically examined. The culture of immature flowers of the same cultivar resulted in the formation of a white nodular callus. Transfer of this callus to a medium containing 0.01 mg/L NAA resulted in differentiation of roots and embryo-like structures with white cotyledonary sheath but no further development of shoot apex.Khartoum University Pres