Lifetime computing algorithms based on exponential pattern retrieve and polynomial fitting in fluorescence lifetime imaging microscopy

How to simulate the decay pattern is crucial during lifetime inversion while utilizing intensity images acquired at increasing delays in time gated fluorescence lifetime imaging microscopy(FLIM) method. A relatively novel understanding of fluorescence decay pattern theory and stimulation algorithms of time gated FLIM method have been analyzed in this paper comprehensively. Main lifetime computing algorithms can be classified as exponential pattern retrieve and polynomial fitting procedure. Especially, a novel lifetime computing method based on bi-exponential decay has been discussed. In experiment, we have validated the proposed algorithms utilizing synthetic images. Performances like calculating precision and computing speed of the algorithms above have also been compared.?2011 SPIE CCC code