Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins

Vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves and other plant materials. (C) 2015 Elsevier B.V. All rights reserved.Vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves and other plant materials. (C) 2015 Elsevier B.V. All rights reserved