Add to ORKG本研究从实验室保藏的节旋藻(Arthrospira)藻种出发,挑取形态不同的单藻丝体进行纯化培养,采用6种方法进行全基因组DNA提取的比较研究,而后以16S rRNA-ITS区基因作为分子标记对藻株进行相关序列测定和分子系统进化分析。结果表明,冻融CTAB法能够提取出包含染色体外DNA在内的节旋藻全基因组,高质量样品可以满足分子生物学实验要求;分子系统研究表明,纯化藻株皆为钝顶节旋藻,节旋藻与螺旋藻在分子鉴定中属间差异明显
The internally transcribed spacer (ITS) sequences of 21 Arthrospira clonal strains from four contine...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
CTAB法提取灵芝菌丝体总DNA,再以核糖体DNA转录间隔区(ITSⅡ、IGR IPCR RFLP)结合随机引物扩增多态性(RAPD)标记分析30个灵芝菌株间的亲缘关系.结果表明,ITSⅡ、IGR I...
利用三種植物DNA抽取套組及三種以CTAB為基礎的萃取方式，萃取兩種非光合作用植物日本蛇菰（Balanophora japonica）和菱形奴草（Mitrastemon kanehirai）的DNA，...
Molecular genetic verification of the taxonomic status of 6 microalgae strains was performed. On the...
甜椒疫病菌Phytophthora capsici之粒線體DNA經由三個RAPD引子增幅後，選取三個增幅片段與載體PCR-ScriptTM Amp SK(+)結合，再轉型至E. coli CL 1菌株...
外瓶霉可致人类感染,不同生物群落的菌种,其致病性、药敏性等特征具有差异性.通过对10株棘状外瓶霉核糖体基因及其转录间隔区进行序列测定,并与GeneBank中9株同种真菌对比分析,揭示了不同生物群落的棘...
エゾコザクラは北海道の高山帯の雪田跡に群生するが，どの自生地でも密度が低く，北海道レッドデータブックで希少種と扱われている。生物多様性を保護するには遺伝子の多様性を把握し，それらに配慮した保全や復元が...
A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
从采自成都地区的中药植物连翘Forsythia suspense和水茄Solanum torvum的根部分离到14株内生放线茵.活性筛选表明,10株菌的发酵粗提物具有不同程度的抗肿瘤活性,占全部菌株的...
本試驗以源自日本、美國、巴西和匈牙利等地區之番椒屬14個品系，探討利用逢機增殖多型性DNA（RAPD）建立種間之分子標誌，以鑑別番椒屬重要野生馴化種之種原。試驗結果顯示，以UBC327，UBC346，...
Here we report the whole-genome shotgun sequence of a Peruvian strain of Arthrospira platensis (Para...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
The internally transcribed spacer (ITS) sequences of 21 Arthrospira clonal strains from four contine...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
CTAB法提取灵芝菌丝体总DNA,再以核糖体DNA转录间隔区(ITSⅡ、IGR IPCR RFLP)结合随机引物扩增多态性(RAPD)标记分析30个灵芝菌株间的亲缘关系.结果表明,ITSⅡ、IGR I...
利用三種植物DNA抽取套組及三種以CTAB為基礎的萃取方式，萃取兩種非光合作用植物日本蛇菰（Balanophora japonica）和菱形奴草（Mitrastemon kanehirai）的DNA，...
Molecular genetic verification of the taxonomic status of 6 microalgae strains was performed. On the...
甜椒疫病菌Phytophthora capsici之粒線體DNA經由三個RAPD引子增幅後，選取三個增幅片段與載體PCR-ScriptTM Amp SK(+)結合，再轉型至E. coli CL 1菌株...
外瓶霉可致人类感染,不同生物群落的菌种,其致病性、药敏性等特征具有差异性.通过对10株棘状外瓶霉核糖体基因及其转录间隔区进行序列测定,并与GeneBank中9株同种真菌对比分析,揭示了不同生物群落的棘...
エゾコザクラは北海道の高山帯の雪田跡に群生するが，どの自生地でも密度が低く，北海道レッドデータブックで希少種と扱われている。生物多様性を保護するには遺伝子の多様性を把握し，それらに配慮した保全や復元が...
A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
从采自成都地区的中药植物连翘Forsythia suspense和水茄Solanum torvum的根部分离到14株内生放线茵.活性筛选表明,10株菌的发酵粗提物具有不同程度的抗肿瘤活性,占全部菌株的...
本試驗以源自日本、美國、巴西和匈牙利等地區之番椒屬14個品系，探討利用逢機增殖多型性DNA（RAPD）建立種間之分子標誌，以鑑別番椒屬重要野生馴化種之種原。試驗結果顯示，以UBC327，UBC346，...
Here we report the whole-genome shotgun sequence of a Peruvian strain of Arthrospira platensis (Para...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
The internally transcribed spacer (ITS) sequences of 21 Arthrospira clonal strains from four contine...
Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chlorofor...
CTAB法提取灵芝菌丝体总DNA,再以核糖体DNA转录间隔区(ITSⅡ、IGR IPCR RFLP)结合随机引物扩增多态性(RAPD)标记分析30个灵芝菌株间的亲缘关系.结果表明,ITSⅡ、IGR I...