Caracterización molecular y citogenética de pacientes con cáncer

El cáncer es un grupo de enfermedades caracterizada por el aumento de la proliferación de una población celular como consecuencia de la acumulación de alteraciones genéticas. En el presente estudio se presenta un aporte a la identificación y validación de los genes HER-2, LMO4 y Mamoglobina como la identificación de diferentes anomalías cromosómicas, a partir del análisis de sangre periférica de pacientes con cáncer (mama, colon, gástrico, pulmón, ovario) y un grupo control.The cancer is a group of diseases characterized by the increase of cell proliferation due to the accumulation of genetic alterations. This study presents a contribution in the identification and validation of the genes HER-2, LM04 and mamoglobin, and the description of chromosome anomalies in the peripheral blood samples with cancer (breast, colorectal, stomach, lung, and ovarian) in comparison with a control group. The level of gene expression was measured by qPCR and the chromosome anomalies were analyzed by cytogenetic conventional techniques. The expression of Her-2 (40 %) and mamoglobin (60 %) was only observed in the breast cancer group. In contrast, the gene expression of LM04 was only observed in both the breast cancer group (65 %) and in the other groups with different types of cancer (58 %).Biólogo (a)Pregrad