小兒の鉄代謝に関する研究 〔その3〕 血色素測定に関する知見補遺

The determination of the accurate value of hemoglobin concentration is a prerequisite in the diagnosis of anemia. Wong (1928) introduced an original method to determine hemoglobin iron which was modified in the present studies due to its lack in accurately determining the value of hemoglobin iron. The modified method is as follows. The iron of the hemoglobin molecule was detached completely by sulfuric acid and potassium permanganate without removing protein: Potassium persulfate and potassium rhodanatum were added and the quantity of iron was measured against the color of a standard solution of known iron content. The results obtained are as follows : The values obtained by the modified method were compared with that of Wong's method and it was found that the latter was lower than the former. The maximal and minimal degree of decrease were 37.2% and 14% respectively (the average, 15%). Hence it may be said that in order to accurately determine hemoglobin iron it is necessary to completely detach iron from the hemoglobin molecule by the action of sulfuric acid and potassium permanganate