Actomyosin溶液の粘度に関する研究 III. 犬の膀胱筋Actomyosinの態度

Actomyosin extracted from bladder muscle of dog was purified, and its viscosity was determined under various conditions in order to compare the same with that from skeletal muscle. The extracting was extremely difficult presumably because of a low content, though the AM extracted was similar in appearance to that from skeletal muscle. Following addition of ATP to myosin-B and to the purified AM, the typical three phases of viscosity change were observed, but in a remarkably light grade as compared with that of skeletal muscle. In the curve of viscosity change of this actomyosin in relation with protein concentration, the structural-viscosity was not as clear cut as in the case of skeletal muscle, however the curve coincides approximately with the latter at other points. ATP-drop, initial viscosity and myosin-content are in lower grades as compared to the latter. The relationship between viscosity and temperature was also the same in quality between the two. The viscosity was at the highest in 0℃, and descended remarkably in accordance with the rise of temperature. As mentioned above, the quality of AM extracted from bladder muscle showed on difference as compared to the AM sample from skeletal muscle and the characteristic differences must be caused by the difference of AM-content of two kinds of muscle