Role of Glucose Metabolism in Multiple Organ Failure caused by Experimental Peritonitis : Glycolytic Intermediates and Energy Metabolism on Rat Liver, Kidney and Heart in Endotoxin Shock

Recently, we have reported the metabolic disturbance of carbohydrate in the liver which appears in septic shock due to peritonitis. The change of glycolytic intermediates and energy metabolism on rat liver, kidney and heart in septic shock was studied in this paper. Liver, kidney and heart tissues were collected 5 hours after induction of peritonitis by cecal incision in fasted male rats. These glycolytic intermediates and adenine nucleotides were assayed by UV-spectrophotometry. The results were summarized as follows; 1. Peritonitis rat group seemed to have coagulopathy and showed slight liver dysfunction and hypoglycemia in chemical blood analysis. 2. Plasma endotoxin concentration was 117.86±62.59ng/ml in the peritonitis rat group. 3. An anaerobic glycolysis increased in the peritonitis rat liver. It was speculated that phosphofructokinase, i. e. glycolytic key enzyme, was activated and fructose-1, 6-diphosphatase, i. e. gluconeogenetic key enzyme, was inhibited. These results suggest that metabolic failure of glucose in septic shock attributed not only to circulatory insufficiency like hemorrhagic shock but also additional reactions. 4. The glycolytic intermediates were not significant in the peritonitis rat kidney. But the energy charge level was significantly lowered compared to the control and it seems that these results were due to cellular hypoxia from a circulatory deficit. 5. The ATP and energy charge level were significantly elevated with increasing glycolysis, which indicated the coronary flow and mitochondrial function maintained in the peritonitis rat heart. They were different from the anoxic and ischemic heart