In the present work a sequential injection system for the enzymatic determination of Image (−)malic acid in wines is described. The determination was based on the detection of NADH, formed by oxidation of Image -malate to oxaloacetate, catalysed by Image -malate dehydrogenase. A kinetic method was implemented by stopping the flow when a solution plug containing sample, NAD+ and enzyme reached the detector. The rate of reaction was measured by monitoring absorbance changes at 340 nm during a fixed period of time. A linear calibration curve (rate of reaction versus concentration) was obtained for concentrations of Image (−)malic acid between 0.01 and 0.15 g l−1. The determination rate was about 22 h−1 and samples were diluted 25 or 50 times b...
The tart-tasting malic acid is one of the key flavour determinants in wine. In winemaking, malic aci...
A flow injection analysis (FIA) system for the spectrophotometric determination of ethanol in wines ...
The tart-tasting malic acid is one of the key flavour determinants in wine. In winemaking, malic aci...
The enzymatic determination of L(-)malic and L(+)lactic acids in several types of wines by flow inje...
A flow-injection system for the enzymatic determination of L( ) lactic acid and L(ÿ) malic acid in w...
A colorimetric method has been developed and optimized to measure L-malic acid in samples of fruit j...
A colorimetric method has been developed and optimized to measure L-malic acid in samples of fruit j...
A colorimetric method has been developed and optimized to measure L-malic acid in samples of fruit j...
A sequential injection system was developed for the enzymatic determination of ethanol in wine. The ...
The malate dehydrogenase (MDH) and ascorbate oxidase were immobilized independently, onto silanized ...
A sequential injection system for the enzymatic determination of ethanol in wines with spectrophotom...
Enzymatic mirocalorimetry has been successfully employed in the reliable determination of the L-mali...
Electroanalytical methods based on biosensor technology and differential PH measurement for the dete...
Electroanalytical methods based on biosensor technology and differential PH measurement for the dete...
This work aims to develop a new enzymatic method for analysing fumaric acid in wines. Fumaric acid i...
The tart-tasting malic acid is one of the key flavour determinants in wine. In winemaking, malic aci...
A flow injection analysis (FIA) system for the spectrophotometric determination of ethanol in wines ...
The tart-tasting malic acid is one of the key flavour determinants in wine. In winemaking, malic aci...
The enzymatic determination of L(-)malic and L(+)lactic acids in several types of wines by flow inje...
A flow-injection system for the enzymatic determination of L( ) lactic acid and L(ÿ) malic acid in w...
A colorimetric method has been developed and optimized to measure L-malic acid in samples of fruit j...
A colorimetric method has been developed and optimized to measure L-malic acid in samples of fruit j...
A colorimetric method has been developed and optimized to measure L-malic acid in samples of fruit j...
A sequential injection system was developed for the enzymatic determination of ethanol in wine. The ...
The malate dehydrogenase (MDH) and ascorbate oxidase were immobilized independently, onto silanized ...
A sequential injection system for the enzymatic determination of ethanol in wines with spectrophotom...
Enzymatic mirocalorimetry has been successfully employed in the reliable determination of the L-mali...
Electroanalytical methods based on biosensor technology and differential PH measurement for the dete...
Electroanalytical methods based on biosensor technology and differential PH measurement for the dete...
This work aims to develop a new enzymatic method for analysing fumaric acid in wines. Fumaric acid i...
The tart-tasting malic acid is one of the key flavour determinants in wine. In winemaking, malic aci...
A flow injection analysis (FIA) system for the spectrophotometric determination of ethanol in wines ...
The tart-tasting malic acid is one of the key flavour determinants in wine. In winemaking, malic aci...