Bioanalytical application of functional magnetic nanoparticle and analytical magnetapheresis

[[abstract]]本論文主要關於製備功能性奈米粒子（functional nanoparticles）並結合“分析磁泳技術”( analytical magnetapheresis ) 做生化分離上之應用研究。主要利用化學共沉澱方法( co-precipitating )製備磁性粒子，再對磁性粒子以高分子修飾粒子表面之官能基為 NH2，最後以各種偵測儀器鑑定磁性粒子之粒徑分佈、吸收等特性。本研究成功製備 40 nm 之 Fe3O4 粒子。磁性粒子經修飾粒子表面後粒徑為80 nm。 本實驗將牛血清蛋白（Bovine serum albumin，BSA）固定在高分子修飾的四氧化三鐵磁性載體上，將蛋白質與顆粒進行共價鍵鍵結；再將抗牛血清蛋白( anti-Bovine serum albumin，anti-BSA )固定在高分子的載體上，結合“分析磁泳技術”（analytical magnetapheresis）做簡單生化分離上之測試研究。藉由帶有 BSA的磁性顆?，可以成功地與帶有 anti-BSA 之高分子顆?作用，探討抗原抗體在磁場作用下的專一性。且製備磁性顆粒並對其作表面改質後與免疫分析系統結合，其具有快速分離、高選擇性、操作簡單及低成本等優點。[[abstract]]The goals of this study are to synthesize functional magnetic particles and to combine with analytical magnetapheresis for biochemical analysis. Magnetic particles ( Fe3O4 ) were prepared by co-precipitating and surface modifications with polymer. Finally, we used variety of detection devices to identify the size distribution, absorption and other characteristics of magnetic particles. The sizes of magnetic nanoparticles were 40 nm. The sizes of polymer coated Fe3O4 were 80 nm. In this study, BSA was binding onto Fe3O4@polymer particles and anti-BSA was bindig onto polymer, and applied to magnetic carriers for bioseparation in analytical magnetapheresis. We explored the specific interaction between antigen and antibody under the magnetic field. The advantages of this methed are rapid separation, high selectivity, simple operation, and low cost.[[note]]碩