(30(3):332-339)The chlamydospore germination of Mycogone perniciosa and its effect on the occurrence of wet bubble of mushroom on the bed

以不同處理方式來促進洋菇腫柄病菌（Mycogone perniciosa Magn.）厚膜抱子發芽，結果顯示以化學藥物處理，溫度處理及酸碱處理均無法促使其在人工培養基中發芽。但若置於充滿洋菇菌絲代謝所產生之揮發性氣體之空間，則於PDA培養基上厚膜孢子在16-28℃均可發芽，而24℃為最適合發芽之溫度，3個月齡期之厚膜孢子為最適合發芽之時期。厚膜孢子在培養24小時內很少發芽，且12℃以下及32℃以上均不發芽，發芽率在1~14%才間；厚膜孢子發芽管由下方之Cell中伸出，可有1支或2支，而後迅速分支或長出一段時間後再分支，約在培養96小時後，厚膜孢子之內含物全部脫離而轉移至發芽管，剩下一空殼。若將厚膜孢子接種於覆土中，則在第加天即可產生具有病徵型之洋菇子實體，且有二種病徵型存在。Chlamydospores of Mycogone perniciosa, the causal organism of wet bubble of mushroom, did not germinate on different media in the laboratory, not even treated with heat (40 to 70°C), chemicals and/or organic solvents. However, the germination was observed on potato dextrose agar connected to the gas produced by the mycelia of mushroom in compost 36 hours at 24°C. The maximum percentage of germination, i. e.14%, was recorded 48 hours at 24°C on 3-month-old chlamydospores. No germination was observed when the temperature was lower than 12 or higher than 32°C. One or two germ tubes were produced from the low cell of chlamydospore. Ninety-six hours after incubation, the cell content of chlamydospore completely moved into the germ tubes leaving an empty cell. When chlamydospores were incorported into the casing soil on the bed, bubble mushroom which is the typical symptom were produced. Therefore, the germination of the chlamydospore of M. perniciosa may be, in part, stimulated by the gas produced by the mycelia of mushroom and/or the complex of the mycelia and the growth media