(45(2):152-163)Studies on Tissue Culture of Angelica sinensis (Oliv.) Diels. ( I ) Induction and Growth of Embryogenic Callus from Immature Embryo

當歸Angelica sinensis (Oliv.) Diels載於本經中品，歷代本草均有著錄，自古即為我國之重要藥材。其功用甚廣，應用於許多中醫方劑，主治婦人疾患。當歸的傳統栽培法是以種子為材料，而種子繁殖的缺點是其品質不易控制，影響當歸品質甚巨。植物組織培養是取代傳統育種及栽培方式，提升當歸品質的良好方法。本研究探討以當歸之未熟胚建立大量繁殖的可行性。結果顯示，當歸未熟胚於MS基礎培養基中培養一個月後，可形成擬胚化癒合組織、正常植物體及不正常植物體三種材料。擬胚化癒合組織於MS基礎培養基之生長狀況較B5及White培養基好。而培養基之無機氮源以NH4+：No3-＝1：2或1：4的組合較適合。此外，25°C與照光（2,000 lux）的條件對癒合組織生長及增殖較佳。Angelica sinensis has been used widely to cute women′s peculiar disease in China. Because of its broad pharmacological effects, it has been adopted in a lot of traditional Chinese medicinal prescription. The purpose of this study is to find the possibility to mass-propagate and improve the quality of A. sinensis by developing its immature embryos. It was found that the embryogenic callus, normal plantlets and abnormal plantlets could be induced from immature embryos cultured on MS basal medium for one month. The growth of embryogenic callus on MS basal medium was better than on B5 and White medium. The inorganic nitrogen source in the medium, NH4+ : NO3- = 1 : 2 or 1 : 4, are the better cdmbina-tion. The embryogenic callus showed better growth when cultured under light (2,000 lux) than in darkness. 25°C was the optimal temperature for the growth of embryogenic callus