(54(1):23-31)Effect of Vegetable and Fruit Homogenates on Seedling Development of Dendrobium moniliforme (L.) Sweet Cultured In Vitro

石斛為我國重要的傳統藥材之一，部份珍貴野生品種已瀕臨絕種，宜建立其組織培養大量繁殖種苗的技術。本試驗以銅皮石斛(Dendrobium moniliforme (L.) Sweet)為材料，在培養基中添加豆薯、馬鈴薯、甘薯及香蕉等蔬果類物質，以進行實生苗繼代培養基配方改良。結果顯示，在含1/2MS 基本鹽類培養基中添加80〜100 g/l 香蕉泥，可增加實生苗株鮮重；小苗生長初期，如直接培養於含80 g/l 豆薯添加物或含20 g/l 甘薯添加物之培養基，能適度增進種苗植株高度；此外，在含有80〜100 g/l 馬鈴薯添加物之培養基中成長的植物體，烘乾後有較高的乾物重，體內蓄積較多的固形物質，可能有利於提高瓶苗馴化移植成活率。Shih-hu is an important traditional Chinese medicine. Due to some of shih-hu were near extinct for protection, an artificial seedling proliferation system for shih-hu is required urgently. An efficient protocol for in vitro propagation and development of D. moniliforme (L.) Sweet using the germinated seedling has been developed. The germinated seedlings (1 cm height) were sub-cultured on half-strength Murashige and Skoog’s (MS) basal medium supplemented with different vegetable and fruit homogenate in various concentrations and 3% sucrose for further growth. The optimum fresh weight of seedlings after three months culturing were found on 80〜100 g/l banana homogenate treatments. The longest height of plantlet were found on 80 g/l yambean homogenate. An increase in dry/fresh weight index was appeared when subculture seedling on the medium supplemented with 80〜100 g/l potato homogenate which might be beneficial for acclimation in vitro seedling