Toxicity of 2,2',4,4',5,5'-hexachlorobiphenyl in the rat-liver: an electron-microscope study

Morphological effects on the liver of Sprague-Dawley rats administered orally 2,2',4,4',5,5'-hexachlorobiphenyl (PCB), congener #153, were analyzed. Treatment diets were prepared by dissolving the congener in 4% corn oil. Ten animals of either sex in each group were placed on the respective diets that contained 50, 500, 5,000, or 50,000 ppb congener. Ten animals of each sex served as the control that had only the oil added to the diets. Thirteen weeks after the onset of dosing, the animals were euthanized and liver samples were obtained from the animals and prepared for electron microscopy. Animals exposed to the congener showed (in a dose-related manner) a marked increase in smooth endoplasmic reticulum profiles, and in the number of lipid droplets in many parenchymal cells. Mitochondrial abnormalities such as dumbbell shapes, and in others, the cristae that were oriented parallel to the long axis of the organelle were present. The magnitude of morphologic alterations did not reveal gender differences. The results indicate mild hepatotoxicity of the congener in the rat.PT: J; CR: AZTORI L, 1991, IND HLTH, V29, P57 BAUMANN M, 1983, ARCH ENVIRON CON TOX, V12, P509 CHEVILLE N, 1976, CELL DEATH DEGENERAT, P44 CHUI YC, 1985, TOXICOL APPL PHARM, V81, P287 CLARKE DW, 1984, CAN J PHYSIOL PHARM, V62, P1253 DURHAM SK, 1989, TOXICOL PATHOL, V17, P536 DURHAM SK, 1989, TOXICOL PATHOL, V17, P782 ELANGBAM CS, 1991, B ENVIRON CONTAM TOX, V47, P321 FISHBEIN L, 1974, ANNU REV PHARMACOL, V14, P139 GHADIALLY FN, 1988, ULTRASTRUCT PATHOL, V2, P767 GILLETTE DM, 1987, FUND APPL TOXICOL, V8, P15 HANSELL MM, 1974, TOXICOL APPL PHARM, V28, P418 JONSSON HT, 1981, ARCH ENV CONTAM TOXI, V10, P171 KASZA L, 1976, J TOXICOL ENV HLTH, V1, P689 KASZA L, 1978, J ENVIRON PATHOL TOX, V1, P241 KIMBROUGH RD, 1972, ARCH ENVIRON HEALTH, V25, P354 LIN FS, 1979, ARCH ENV CONTAM TOXI, V8, P321 MACLELLAN K, 1994, HISTOL HISTOPATHOL, V9, P453 MACLELLAN K, 1994, J SUBMICR CYTOL PATH, V26, P279 NISHIZUMI M, 1970, ARCH ENVIRON HEALTH, V21, P620 NORBACK DH, 1972, ENVIRON HEALTH PERSP, V1, P137 SAFE S, 1984, CRC CRIT R TOXICOL, V13, P319 SAFE S, 1990, CRIT REV TOXICOL, V21, P51 SATO T, 1968, J ELECTRON MICROSC, V17, P158 SCHECTER A, 1984, BANBURY REPORT, V18, P177 SCHECTER A, 1985, ENVIRON HEALTH PERSP, V60, P241 SINGH A, 1981, PATHOLOGY, V13, P487 WEISS L, 1988, CELL TISSUE BIOL TXB, P1; NR: 28; TC: 12; J9: HISTOL HISTOPATHOL; PG: 8; GA: NY622Source type: Electronic(1