An Attempt Directed toward Enhanced Shape Selectivity in Reversed-Phase Liquid Chromatography: Preparation of the Dodecylaminated β-Cyclodextrin-Bonded Phase

With the aim of preparing a stationary phase with a high shape-recognition ability for liquid chromatography, a new bonded phase was synthesized by coupling multiply dodecylamino-substituted β-cyclodextrin (β-CD) to 3-glycidoxypropyl-derivatized silica gel. The stationary phase prepared in this way was expected to have increased shape selectivity compared with that of conventional reversed-phase materials, due to solute interactions with the alkyl chain piles built up on the β-CDs bonded to silica. The separation characteristics of the bonded phase were investigated using polycyclic aromatic hydrocarbons (PAHs) with different molecular shapes and compared with those of monomeric ODS and native β-cyclodextrin-bonded phases. The newly developed stationary phase was found to be highly selective for PAHs