SIMPLE EXTRACTION OF ORGANOPHOSPHATE PESTICIDES USING SOLID-PHASE MICROEXTRACTION (SPME) BEFORE CAPILLARY GAS CHROMATOGRAPHY

A simple method, for extraction of 9 organophosphate pesticides from human whole blood and urine, using the headspace solid-phase microextraction (SPME) method is presented. After heating a vial containing the samples and the pesticides (IBP, methyl parathion, fenitrothion, malathion, fenthion, isoxathion, ethion, EPN and phosalone) at 100°C , a polydimethylsiloxane-coated SPME fiber was exposed to the headspace of the vial to allow adsorption of the pesticides before a capillary gas chromatography (GC) with nitrogenphosphorus detection. The headspace SPME-GC for whole blood and urine gave peaks for each pesticides. Recoveries of the 8 pesticides were 11-0.3% for whole blood; those of the 9 pesticides were 40-3.8% for urine. The detection limits of each pesticide were 2.2-100 ng/0.5ml for whole blood and 0.8-12 ng/0.5ml for urine. Reproducibility for headspace SPME-GC data were excellent for both body fluids.rights:日本法中毒学会rights:本文データは日本法中毒学会の許諾のもと掲載しています