The development of fertility treatment for patients with premature ovarian failure or diminished ovarian reserve by regulating activin action.

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 2011～2013課題番号: 23592400研究代表者: 木村 文則（滋賀医科大学・医学部・講師）出生後5.5日に摘出した卵巣にプロゲスチンを添加し48時間培養すると、卵巣内のActivinβAおよびActivinβB共に半分程度に抑制された。16週齢のメスICRマウスに5㎎のディナゲスト（DNG）を4日毎の皮下注射を16回行った後、交配させると生涯総分娩仔数はDNG群の方が非投与群より多かった。最終分娩週齢もDNG投与マウスで高かった。卵巣内のアクチビンを抑制し、原子卵胞維持に働いた可能性がある。晩婚化が進む本邦において卵巣機能温存し、高齢でも妊孕性を維持する方法になりうると考えられる。Mouse neonatal ovaries of day 5.5 were cultured with or without progestin for 48 hours. We found the Activin beta A and beta B mRNA expression was decreased to half. Based on the fact we researched the effect of continuous progestin exposure to mouse ovarian function. Five mg of dienogest solved in corn oil or corn oil was given by intraperitoneal injection to sixteen week old mice every four days. After sixteen times injection the mice were housed with male mice. The total numbers of pups born from a mouse were compared. It was greater in dienogest group (P < 0.03). The last delivery age was also higher in dienogest group. We speculate that dienogest suppressed primordial follicle activation and maintained the primordial follicle pool through the suppression of activin action. This result would be applicable for the maintenance of ovarian reserve and fertility in the developed country including Japan, where women get married in older age. Now we investigate this progestin effect